Why do you hold the pipet in a

Blood dilution topic:

1. Why do you hold the pipet in a nearly horizontal position during dilution?
2. What are the factors that may affect the accuracy of dilutions made with blood diluting pipets?
3. List down the expected errors that can be encountered in the dilution method.
4. What is the dilution factor if the blood is sucked to 0.4 mark of RBC pipet?

please attach reference book on this topic after the last question

Hemacytometer topic:

1. what is the distance between the cover slip and the counting chamber?
2. What is the total white blood cell area?
3. What is the total red blood cell area?

please attach reference book on this topic after the last question

Red Blood cell count topic:

1. Why do you discard the first four drops of the diluting fluid drawn up to 101 mark from the pipet?
2. Name the different RBC diluting fluids.
3. Why is Formol-citrate the best RBC diluting fluid?
4. In case of polycythemia, the count is 8.0 x 10¹²/L, Explain the variation in technique.

please attach reference book on this topic after the last question